BD offers a fully functional 30-day trial of FlowJo v10 for Windows and Mac. This is ideal for a short-term project or to test compatibility before purchase. Register on the official website to receive a temporary license key.
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If FlowJo remains out of reach, consider Flowing Software (free for non-commercial use) or CytoBank (a free cloud-based platform with limited storage). R/Bioconductor packages like flowCore and openCyto offer powerful, scriptable analysis—completely free, though requiring programming knowledge. BD offers a fully functional 30-day trial of
: Many universities and research facilities maintain "dongles" (USB hardware keys) that can be checked out by researchers for short-term use at no cost. scriptable analysis—completely free